Our Patented Camelina Transformation System using a Non-selection Technique
SOURCE MATERIAL
Our source plants are grown in vitro from sterilized seeds that are produced in a greenhouse.
INOCULATION
Leaves from the source plants are cut and placed on filter paper that has been soaked in a liquid culture of Agrobacterium with construct.
CO-CULTIVATION
These explants are then grown in an MS agarised medium containing 0.5-3% sucrose, BAP, and NAA.
CALLUS FORMATION
Transgenic inclusions appear in the form of a callus after about one week of cultivation in the same medium as above with the addition of carbenicillin to prevent agrobacterium growth.
SHOOT REGENERATION
After two weeks of growth in a mixture of MS agar containing BAP and 3% of sucrose and carbenicillin, the explants have produced several viable shoots.
ROOTING
These shoots are cut from the callus and transferred to a mixture of MS agar containing NAA and 3-4% sucrose. This mixture promotes rooting.
TRANSGENIC PLANTS
Once transgenic plants have grown from the shoots, they are transferred to soil and placed in a greenhouse.